Mutations in the Notch1 gene from tumors developed in Id1 and Hes1 single or double transgenic mice.

<p>DNA samples isolated from individual tumors from indicated transgenic strains were used as templates to amplify the sequence encoding the PEST domain of Notch1. PCR products were cloned and sequenced as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006678#s2" target="_blank">Materials and Methods</a>. (A) Sequence of the mutations. Wild type Notch1 sequence in the relevant regions is shown in blue and amino acid numbers for each region are indicated. Mutant sequences from the tumors are aligned underneath and the type of the mutation is indicated at the end of each sequence (FS = frame-shift, Stop = nonsense mutation). (B) Frequency of Notch1 mutation. Numbers of tumors containing mutations and those with wild type sequence are depicted with black and blue bars, respectively.