Human albumin is not expressed in mouse liver engrafted with pediatric MSC and adult MSC.

<p>A) Staining with anti-human albumin Ab did not detect any albumin positive cells within mouse liver parenchyma (a,b,c). Scale bars indicate magnification. B) Total RNA was extracted from sham injected or transplanted mouse liver. Expression of human vimentin, human αFP and human albumin was analyzed by RT-PCR in several liver tissues after intra-hepatic transplantation with human MSC. Sham: sham injected mouse liver. C+: positive control for human vimentin was RT-PCR on MSC extract. Positive control for αFP and albumin was RT-PCR on human hepatocytes extract. C-: negative control without polymerase. Vimentin was expressed in liver tissue demonstrating engraftment of MSC. Human αFP and albumin were never detected. Each figure shows one representative result of several independent experiments (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006657#pone-0006657-t001" target="_blank">table 1</a>).