Co-localization of AR, GnRH, and CRH in MPO neurons, determined by dual-fluorescence immunohistochemistry and confocal laser-scanning microscopy.

<p>A) Main distribution of AR, GnRH, and CRH in control female rat brain (3v = third ventricle). Adapted from reference <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0006638#pone.0006638-Paxinos1" target="_blank">[47]</a>. B–D) Co-localization of AR, GnRH, CRH, and NeuN immunoreactivity in MPO neurons. E) Co-localization of rabbit polyclonal AR antibody and mouse monoclonal AR antibody in hypothalamic MPO. F and G) Co-localization of mouse monoclonal AR antibody with GnRH or CRH immunoreactivity in hypothalamic MPO neurons. In panels B–G, arrows indicate co-localization. Scale bars, 100 µm.