Akt dysfunction in the very aged soleus muscle can be corrected by acetaminophen.

<p>A. Akt kinase activity as measured in soleus muscles from 6-, 27-, 33-month control (33C) and acetaminophen-treated (33T) rats. The relative abundance of GSK3 fusion protein phosphorylated by the immunoprecipitated Akt was normalized to total Akt. Data are mean±SE (n = 3). abc: Groups without the same letter are significantly different (P<0.05). B. Soleus muscles from acetaminophen-treated (33T) and age-matched control (33C) animals were <i>ex vivo</i> incubated with insulin (+). Phosphorylation of GSK3α (Ser21), GSK3β (Ser9) and Akt (Ser473 and Thr308) after <i>ex vivo</i> insulin incubation were detected by immunoblotting. Muscle incubated with Krebs Henseleit buffer (−) was used as control of insulin response.