7-AMC adducts detected in <i>wt B. cereus</i> conditioned supernatants.

<p>Wild type <i>B. cereus</i> 569 spores were resuspended in 200 µl TMB buffer to OD₅₈₀ = 1. Spores were treated with 0.2 mM inosine and supernatants were collected 30 min post-inosine addition. Collected supernatants were labeled with 7-AMC. 7-AMC adducts sere separated by RP-HPLC and identified by mass spectrometry. Concentrations were calculated by fluorescence spectroscopy.