Molecular characterization of the <i>T55</i> transgene integration site.

<p>(A) Fluorescent in situ hybridization to metaphase chromosomes prepared from <i>T55</i> splenocytes showing transgene localization to chromosome 16 B2–B4. (B) Map of the <i>T55</i> integration site (top) and endogenous locus (bottom) showing the structure of the 3′ end of the transgene array (black box, <i>A17 Myf5</i> enhancer; blue box, <i>nlacZ</i> reporter gene) and the position of the inverse PCR primers (red arrowheads); B, <i>Bam</i>HI; Bg, <i>Bgl</i>II. Also shown are the flanking sequence isolated by inverse PCR (red) and the Southern blot probe (grey) used to identify the predicted 5.5 kb <i>BamHI</i> fragment in transgenic DNA in addition to a 3.8 kb wildtype fragment (C). (D) Ensembl map of the genomic region surrounding the integration site (red arrow) on chromosome 16, showing the position of the flanking genes <i>Opa1</i> and <i>Hes1</i> and intermediate gene predictions based on EST alignments.