PP6R proteins partially restore zymocin sensitivity to the quadruple <i>sap</i> mutant strain.

<p>Isogenic WT (MLY41) transformed with vector, the quadruple <i>sap</i> mutant (JRY40), and only <i>SAP4</i> (<i>sap155 sap185 sap190</i>; JRY45) strains transformed with vector or constructs expressing <i>PP6R1</i>, <i>PP6R2</i>, <i>PP6R3</i>, or <i>SAP185</i> were grown overnight in SD-Ura medium. Equivalent numbers of cells were serially diluted, and aliquots were spotted onto plates of YPD and YPD containing zymocin. After 3 days of incubation at 30°C, the plates were photographed.