Acetylation status of the MYC locus on human chromosome 8 residing in chicken DT40 cells and in murine B78 cells.

<p>DNA recovered from chromatin immunoprecipitations with antibodies specific for lysine9/14 acetylated histone H3 (H3K9/14ac) was subjected to duplex PCR using primers specific for the human, chicken and mouse MYC genes. The enrichment of these regions in the immunoprecipitated DNA was normalized to the signal detected with primers specific for the endogenous chicken β-globin gene or the mouse β-globin. Upper graph, comparison of histone acetylation levels at the human MYC promoter regions L, K, N and the chicken MYC promoter region in DT40 cells. Primer location and CTCF sites N and A of the human MYC promoter region are shown schematically at the top. Lower panel, comparison of histone acetylation levels at human MYC promoter regions L, K, N, and the murine MYC promoter region after transfer of human chromosome 8 into mouse B78 cells.