Membrane affinity analysis of OmpL36, OmpL37, OmpL47 and OmpL54.

<p>Membrane fraction of <i>L. interrogans</i> was treated with lysis buffer as a control or 0.1 M Na₂CO₃ (pH 11), 1.6 M urea, or 0.6 M NaCl for 15 min at 4°C. Samples were pelleted by centrifugation to separate the pellets (P) and supernatants (S), followed by gel electrophoresis (Bis-Tris 4–12% NuPage gel, Novex), and immunoblotting with specific antisera. The identities of individual proteins are indicated on the right, and the positions of molecular mass standard (in kilodaltons) are indicated on the left.