20(OH)D3 increases mRNA levels of IκBα in keratinocytes.

<p>HEKn and HaCaT keratinocytes were treated with 100 nM 20(OH)D3 or 1,25(OH)₂D3 or vehicle for the indicated period of time. Cells were then lysed and total RNA extracted. mRNA levels for IκBα were measured using reagents for RTPCR according to the manufacturer's protocol (Roche Applied Science, Manheim, Germany) and normalized relative to Cyclophylin B RNA. Data are presented as mean±STD (n = 3) *p<0.05 versus control, or **p<0.01 versus control. Time response in the expression of mRNA was shown for NFκBI (IκBα) in both cells.