Boric acid treatment lowers luminal storage [Ca²⁺]_st, but has no effect on cytoplasmic [Ca²⁺]_cyt in DU-145 cells.

<p>A. Relative [Ca²⁺]_cyt following exposure to 0, 10 and 50 µM BA for 24 hrs (n = 5, NS). B. [Ca²⁺]_st as percent of 0 treatment in DU-145 cells treated with 0–250 µM BA for 24 hrs. Boric acid [10–50 µM] reduced ER calcium levels by more than 22%–32% compared to untreated cells (n = 5,*p<0.05, **p<0.01, ***p<0.001). C. Reduction of [Ca²⁺]_st in DU145 cells treated with 50 µM BA from 1–72 hours (n = 5, **p<0.01). D. Confocal measurement showed pretreatment of cells with BA for 24 hrs lowered thapsigargin stimulated Ca²⁺ release compared to untreated cells (n = 6, ***p<0.0001).