Expression Pattern of <i>bub-1</i>.

<p>(A) P<i>bub-1::GFP</i> was widely expressed in the embryonic stage: (a) early <i>C. elegans</i> embryos in the gonad of an adult animal; (b) comma stage embryo; (c) two-fold stage embryo; and (d) three-fold stage embryo. The scale bar represents 50 µm for (a) and 10 µm for (b), (c), and (d). (B) Antibody staining of BUB-1 in embryo nucleus. (e) DAPI staining and (f) antibody staining of BUB-1 of the same embryo. (g) DAPI staining and (h) antibody staining of BUB-1 of the same embryo. In (e) and (f), some of the cells (as shown by the arrow) were at the prophase of mitosis and BUB-1 was localized in the nuclei. The arrowheads show the non-dividing cell where BUB-1 was not expressed. In (g) and (h), one cell (as shown by the arrow) was at the metaphase of mitosis and BUB-1 was localized to the kinetochore. The scale bar represents 10 µm.