Exposure of mouse PBMCs to trypanosomes results in cell death.

<p>Forward (FSC) and side (SSC) scatter dot plots (upper panels) and corresponding frequency distribution of PI incorporation among mock- and killed <i>T. evansi</i>-exposed PBMCs. P1 (red): annexinV/PI double-negative PBMCs; P2 (green): annexinV/PI double-positive cells with ∼intact FSC/SSC; and P3 (blue) annexinV/PI double-positive PBMCs with a skewed FSC profile suggesting size reduction. A stepwise process is obvious, with size preservation and moderate PI incorporation first, followed by marked PI incorporation and a drastic size reduction at a later stage. Relative population sizes are shown in the three top panels.