Tat-SF1 depletion does not affect pseudotyped HIV-1 replication.

<p>(A) VSV-G replication assay. Serial dilutions of pseudotyped virus (VSV-G envelope) were used in a single-round replication assay with T-Rex-293 cells expressing an empty vector, an shRNA targeting GFP, or shRNAs targeting Tat-SF1. A western blot confirming knockdown is shown below the chart. Luciferase values of the cell lysates were read after 24 hours. Luciferase values were background corrected and normalized to protein content. (B) AMLV replication assay. An AMLV-pseudotyped virus was used in the same type of experiment described in (A) except that lysates were harvested after 48 hours. Values reported are the means of triplicate wells. Error bars represent standard error. The western blot in (A) also corresponds to the cells used in this experiment. (C) VSV-G-replication assay. Pseudotyped virus was used on HeLa cells stably expressing a non-silencing shRNA or a third shRNA targeting Tat-SF1. Luciferase values of the cell lysates were read after 24 hours. Luciferase values were background corrected and normalized to protein content. Values are reported as the means of triplicate wells. Error bars represent standard error.