Anti-IL-10 restores T cell activation by MoDCs.

<p>A. The allostimulatory capacity of MoDCs from FMDV-infected swine (D1 to D17 pi) and naïve swine is evaluated in the presence of anti-IL-10 monoclonal Ab (gray squares) or an irrelevant antibody (white squares) (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005659#s4" target="_blank">material and Methods</a>). MoDCs were irradiated and used as stimulator cells for allogeneic CD3⁺ T cells at the T cell/DC ratio indicated. Proliferation was measured in cpm after [³H]Thymidine incorporation. Data is representative of three independent experiments. B. IL-10 production by co-cultures of MoDC from FMDV-infected swine and T cells from a naïve pig. 72 h after the onset of the co-culture (MoDC: CD3⁺ T cell ratio of 1∶10), supernatants were collected and analyzed for IL-10 production by quantitative ELISA. The figure shows mean concentration values (pg/ml). C. IL-10 production by MoDCs from FMDV-infected swine. IL-10 production by cultures of MoDCs was determined by quantitative ELISA. It is expressed in pg/ml±SD. D. IL-10 produced in sera from FMDV-infected swine at different times post-inoculation. IL-10 was detected by ELISA. Each bar corresponds to one animal and it is expressed as pg/ml±SD. E. IFN-α produced in sera from FMDV-infected swine at days 1, 3, 5, 10 and 17 post-inoculation by ELISA. N, indicates naïve animals. Each bar corresponds to one animal and it is expressed as pg/ml±SD.