IFNγ ELISA analyses after restimulation with immunodominant and latency-associated antigens of PBMC from LTBI and TST-negative donors.

<p>Analyses of IFNγ in the culture supernatant by ELISA after 7 days and two rounds of <i>in vitro</i> restimulation in PBMC from LTBI (<i>A</i>) and TST-negative donors (<i>B</i>) are shown. Scatter plots indicate mean and standard deviation. Background values of non-stimulated controls were subtracted. IFNγ concentrations in the supernatant are indicated on the y-axis for stimulation with SEB, PPD from <i>M. tuberculosis</i>, and tested antigens (x-axes). The most promising candidate Rv3407 is underlined.