PBMCs from immunized animals produce IFN-γ or IL-2 in response to ZEBOVGP peptides.

<p>ZEBOVGP-specific IFN-γ or IL-2 secreting cells from PBMCs were detected by either an (A) IFN-γ or (B) IL-2 ELISPOT assay. PBMCs were obtained from cynomolgus macaques immunized with VSVΔG/ZEBOVGP either orally (OR; n = 4), intranasally (IN; n = 4), or intramuscularly (IM; n = 2). The control NHPs (n = 2) were immunized IM with the heterologous VSVΔG/MARVGP. PBMCs were incubated with 1.5 µg/ml of peptides spanning the entire ZEBOV glycoprotein. Bars represent the average number of ZEBOVGP-specific IFN-γ or IL-2 secreting cells detected in each immunized group.