<p>(<i>A</i>) BrdU was pulsed into MK cells at the indicated times following release from quiescence to determine the kinetics of synchronization and entry into S-phase. (<i>B</i>) In parallel with the BrdU-pulsed samples in A, MK cells were collected at the indicated times and separated into total cell lysates (TCE), or fractionated into nucleosolic/cytosolic detergent-soluble extracts (S1) or chromatin-bound detergent-resistant extracts (P3). Immunoblotting with the indicated antibodies was performed on lysates from equal cell numbers loaded into each lane. The G<sub>1</sub>-S transition in MK cells (12 hrs after release) is overlayed in gray.</p
In this study, a new parameter, S phase cell percentage (S fraction) normalized BrdU (SFN-BrdU) inco...
Passage through the Retinoblastoma protein (RB1)-dependent restriction point and the loading of mini...
Lymphocyte multiplication can be induced in vitro by mitogens or specific antigens, and is usually m...
<p>(<i>A</i>) Parallel to the BrdU and flow cytometry collection in <a href="http://www.plosone.org/...
<p>A) Cells were synchronized by a modified double-thymidine block then released by re-plating and h...
International audienceEukaryotes duplicate their chromosomes during the cell cycle S phase using tho...
<p>(<b>A</b>) G<sub>0</sub> arrested cells were cytospinned on coverglasses, which caused aggregatio...
<p>HeLa or Dox treated p017-4 cells were transfected with control or cyclin B2 siRNA (B2 kd) for 18 ...
<p>A549 cells were pulsed with BrdU for 30 minutes following growth on soft or stiff gels for 2 days...
<p>Cell-cycle kinetics of cells arrested in G1 phase and released into the cell cycle. Samples were ...
<p>A. Cdt1 degradation and chromatin association of proteins. HeLa cells synchronized in M phase wer...
<p>A) Representative flow cytometry data of nucleofected cells that were cultivated for 24 hours, pu...
<p>(<b>A</b>) MDA-MB-468 cells treated with Gltn for indicated times were synchronized in G2/M using...
<p>Microscopy pictures of cells double-labelled with EdU and BrdU. Cells synchronized in G1 phase we...
Carboxyfluorescein diacetate succinimidyl ester (CFSE) labelling has been widely used to track and s...
In this study, a new parameter, S phase cell percentage (S fraction) normalized BrdU (SFN-BrdU) inco...
Passage through the Retinoblastoma protein (RB1)-dependent restriction point and the loading of mini...
Lymphocyte multiplication can be induced in vitro by mitogens or specific antigens, and is usually m...
<p>(<i>A</i>) Parallel to the BrdU and flow cytometry collection in <a href="http://www.plosone.org/...
<p>A) Cells were synchronized by a modified double-thymidine block then released by re-plating and h...
International audienceEukaryotes duplicate their chromosomes during the cell cycle S phase using tho...
<p>(<b>A</b>) G<sub>0</sub> arrested cells were cytospinned on coverglasses, which caused aggregatio...
<p>HeLa or Dox treated p017-4 cells were transfected with control or cyclin B2 siRNA (B2 kd) for 18 ...
<p>A549 cells were pulsed with BrdU for 30 minutes following growth on soft or stiff gels for 2 days...
<p>Cell-cycle kinetics of cells arrested in G1 phase and released into the cell cycle. Samples were ...
<p>A. Cdt1 degradation and chromatin association of proteins. HeLa cells synchronized in M phase wer...
<p>A) Representative flow cytometry data of nucleofected cells that were cultivated for 24 hours, pu...
<p>(<b>A</b>) MDA-MB-468 cells treated with Gltn for indicated times were synchronized in G2/M using...
<p>Microscopy pictures of cells double-labelled with EdU and BrdU. Cells synchronized in G1 phase we...
Carboxyfluorescein diacetate succinimidyl ester (CFSE) labelling has been widely used to track and s...
In this study, a new parameter, S phase cell percentage (S fraction) normalized BrdU (SFN-BrdU) inco...
Passage through the Retinoblastoma protein (RB1)-dependent restriction point and the loading of mini...
Lymphocyte multiplication can be induced in vitro by mitogens or specific antigens, and is usually m...