Induction of FITC-specific CHS in mice adoptively transferred with FITC⁺ MGL2⁺ DDC.

<p>(A) The left panels show the gating to sort FITC⁺ MGL2⁺ cells from the MACS-purified CD11c⁺ cells. The purity of FITC⁺ MGL2⁺ DDCs was approximately 98%. Ear swellings in mice transferred with FITC⁺ MGL2⁺ DDCs (“MGL2⁺”, <i>p</i><0.005), transferred with glutaraldehyde-fixed FITC⁺MGL2⁺ DDC (“fixed MGL2⁺”, difference not statistically significant), sensitized directly with FITC painting (“FITC painted”, <i>p</i><0.005), or untreated (“naive”) are shown in the graph. Each bar indicates mean±SD obtained from three independent recipients respectively. (B) The left panels show the gating strategy to sort FITC⁺MGL2⁺DDCs from the day 1 LNs or FITC⁺MGL2⁻DCs from the day 4 LNs. The purity of sorted cells was >90% when re-analyzed by FACS. Ear swelling in mice transferred with 5×10⁴ cells of FITC⁺MGL2⁺DDCs from the day 1 LNs (d1 DDC), glutaraldehyde-fixed FITC⁺MGL2⁺DDCs from the day 1 LNs (fixed d1 DDC) or FITC⁺MGL2⁻DCs from the day 4 LNs (d4 non-DDC) is shown in the right panel. The mice sensitized by FITC painting (FITC painted) and left untreated (naive) were used as positive and negative controls respectively. Each bar indicates the mean value from two recipients and the data are representative of two independent experiments.