Bimodal distribution of adipocytes in Nscl-2 (−/−) mice and normal <i>in vitro</i> differentiation of isolated Nscl-2 mutant preadipocytes.

<p>(A) Semithin sections (Richardson stain) of epididymal adipose tissue from 6 month old male animals. Histograms indicate the size distribution of isolated adipocytes from different strains. Scale bars: 100 µm. (B) FACS analysis (sideward versus forward scatter) of preadipocytes after 1 day in culture reveals a general uniformity in cell size and granularity. (C) Normal <i>in vitro</i> differentiation of isolated Nscl-2 mutant preadipocytes. Differentiated, lipid drop containing cells were identified by oil red staining. (D) Relative mRNA expression level of adipocyte differentiation related genes and adipocytokines expressed in epididymal WAT determined by quantitative Real-Time PCR (mean values, n≥3). Values in bold indicate changes >150% compared to controls, values in italic indicate changes <50% of compared to controls. Relative expression level = (GeneX^mutant/36B4^mutant)/ (GeneX^WT/36B4^WT).