Comparative alignment between VACV G8R, human and yeast PCNA.

<p>Alignment created using T-Coffee alignment algorithm, default parameters, with minor adjustments made by hand <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005479#pone.0005479-Notredame1" target="_blank">[20]</a>. Green arrows represent beta-strands, and red cylinders represent alpha-helices. Black rounded box shows the interdomain connecting loop, blue shaded boxes represent the helices that contribute to DNA binding and dots represent identical residues between the 3 protein sequences. Residue marked with an asterisk is the conserved ubiquitylation/SUMOylation site. Secondary structure was derived directly from the crystal structures of the human and yeast PCNA proteins and the structural model of the VACV G8R protein.