Immunofluorescent co-localization of RyR2 and L-type Ca²⁺ channels in hESC-CMs.

<p>A. Volumetric view of a stack of confocal anti-Cav1.2 images from a representative hESC-CM. Images were collected every 0.25 µm in the z-axis. The scale bars in the image indicate 5, 2, and 5 µm along the x, y, and z-axes, respectively. The horizontal grey boxes show the location of the two optical sections depicted in panel B. B. Two-dimensional anti-RyR2 (green, left), anti-L-type Ca²⁺ channels (anti-Cav1.2, red, center), and merged images, corresponding to optical sections 1 and 2 from panel A. Note that the merged image from optical section 1 includes two dotted lines, one that runs along the cell membrane (magnified in the inset) and one that traverses the cell. The corresponding plots to the right indicate the two-channel fluorescent intensity along each of the aforementioned lines.