Circulating mature B cells and kinetic analysis of peripheral blood B and T cell depletion.

<p>Upper graph: C57BL/6 mice were injected i.v. at day 0 with 0.5 mg of a given anti-BAFF-R antibody, as indicated. At day 14 the percentage of CD93⁻CD19⁺ mature B cells was determined by FACS analysis on the peripheral blood mononucleated cells. Each symbol represents an individual mouse. Statistical analysis revealed a significant difference for control versus 9B6, 9B9 and 5H10 (p<0.05). Lower graph: C57BL/6 mice were injected with either 0.5 mg of the anti-BAFF-R mAb 9B9 (white bars), or 0.5 mg of the anti-CD4 mAb GK1.5 (grey bars). Black bars represent PBS injected controls. The mAbs were injected at day 0 and the percentages of immature B cells (CD93⁺CD19⁺), mature B cells (CD93⁻CD19⁺), CD4 and CD8 T cells were determined by FACS at days 4, 7 and 10. Each column represents a time point: day 4 left column, day 7 middle and day 10 right column. Statistical analysis revealed a significant difference between PBS treated mice as compared to anti-BAFF-R mAb at each time point analyzed for mature B cells but not for immature B cells.