Depletion of the B and T cell subsets following anti BAFF-R 9B9 antibody injection.

<p>Panel A. Representative FACS plot of the immature (CD93⁺CD19⁺) and mature (CD93⁻CD19⁺) B cell compartments in the spleen of C57BL/6 mice; upper plots untreated control, lower plots day 14 after 0.5mg of anti-BAFF-R 9B9 injection. On the left side total splenocytes are depicted. On the right side, gated on mature B cells (CD93⁻CD19⁺) follicular (CD21⁺CD23⁺) and MZB (CD21^highCD23^low) B cells are shown. Panel B. Absolute numbers of splenic T1 and T2/3 immature B cells, B-2 and MZ B cells, CD4 and CD8 T cells in controls (black bars) and 9B9 injected C57BL/6 mice at day 14 after injection (white bars). 5 mice were analyzed for each group. A significant difference could be observed for T2/T3, B-2 and MZB cell numbers in control as compared to 9B9 injected mice. Panel C. Representative FACS plot analysis indicating the percentages of CD19⁺CD23⁺ B-2, CD19⁺CD11b⁺ B-1b and CD19⁺CD5⁺ B-1a B cells in the peritoneal cavity of control (upper dot plots) and C57BL/6 mice injected with 9B9 mAb at day 14 after injection (lower dot plots).