The amino acid sequence of the PmpD fragments used to raise antibodies and antibody reaction with proteins present in the soluble fraction.

<p>A. The amino acid sequence of the PmpD fragments used to raise antibodies (Fragment 1, aa 17–517; fragment 2, aa 470–818; fragment 3, aa 819–1180; fragment 4, aa 1174–1531). B. Reaction of antibodies raised against PmpD fragments with proteins present in the soluble fraction. McCoy cells infected with <i>C. trachomatis</i> serovar L2 were harvested at 48 h p.i. and the soluble fraction was prepared as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005191#s2" target="_blank">METHODS</a>. Lane 1, pAb against fragment 1. Lane 2, pAb against fragment 2. Lane 3, pAb against fragment 3. Lane 4, pAb against fragment 4.