Up-regulation of the IDA-1::GFP translational reporter gene in normal and ectopic neurons in <i>gv560</i> mutant animals.

<p>(A) Schematic diagram of the integrated IDA-1::GFP translational fusion reporter transgene in strain KM246 that was used for genetic screens. SP, signal peptide; TM, transmembrane domain; PTP, protein-tyrosine phosphatase-like domain. (B) Schematic diagram of a <i>C. elegans</i> hermaphrodite showing the positions of relevant cell bodies from the left lateral view with anterior to the left and dorsal to the top. (C) Ventral view of IDA-1::GFP transgene expression in VC4, VC5, VC6, PHC, and HSN neurons in wild type animals. (D) IDA-1::GFP levels in <i>gv560</i> mutant animals is markedly increased in VC4 and VC5 neurons and also is observed in four additional neurons (P9–P12 descendants) in the posterior region. (E) IDA-1::GFP in the cell body of the ALA neuron in the head of a wild type (left panel) and <i>gv560</i> mutant (right panel) animal; images were collected with identical exposure times. (F) P<i>_lin-11</i>GFP reporter expression in VC1-6 cells in a wild type animal. (G) P<i>_lin-11</i>GFP reporter expression confirmed that the additional posterior neurons observed in <i>gv560</i> mutants are derived from P9–P12. (H) IDA-1::GFP expression is observed in extra neurons derived from P9–P12 in <i>ced-3</i> mutant animals, but, it is not upregulated in VC or HSN neurons (only HSN(R) is visible in this focal plane). (I) Average intensities of IDA-1::GFP for five VC4 (left) or VC5 (right) neurons in each strain were quantitated and compared between wild type controls and <i>gv560</i> or <i>ced-3(n717)</i> mutants. **, P<0.01. All images are of adult animals oriented with anterior to the left and (where possible) dorsal towards the top. Scale bars indicate 40 µm.