Silencing c-Rel resulted in diminished cell survival and cell cycle progression in Wehi-231 cells.

<p>(a) 2 ml of Wehi-231 cells (2×10⁶/ml) were cultured in a 6-well plate with anti-CD40 (10 µg/ml) for 48 hours in the presence of polybrene (4 µg/ml) and different dosages of the c-Rel siRNA expressing retrovirus or the control virus (0.3125, 0.625, 1.25, and 2.5×10⁶). Cells were harvested and monitored by flow cytometry for the percentage of GFP⁺ cells. c-Rel silencing was confirmed by western blot analysis. (b) Cell survival and cell cycle progression was analyzed by PI staining. The number of percentage in M1 indicates the percentage of the analyzed cells undergoing apoptosis. The number in M2 representing the number of cells entering into cell cycle. (c) Data in (b) were summarized as line chart type figure.