Mediator association at putative target and non-target regions in SC and YPD medium.

<p>(A) Association of Med15-(HA)₃ was determined at the indicated genomic regions as described previously <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005029#pone.0005029-Fan1" target="_blank">[10]</a>. Relative occupancy values were calculated by determining the apparent immunoprecipitation efficiency (ratio of immunoprecipitated to input DNA) and normalized to the average value of 10 non-target regions (underlined with dashes), which was defined as 1 and served as the internal control. All values represent the mean of at least three independent experiments and the standard deviation is indicated. The indicated ranks of genomic regions are taken from Andrau et al. (2006) by sorting by Mediator.BR descending of the 1241 targets (gene names: p, promoter regions; o, ORF regions) and the rest of non-targets separately. (B) Control experiment with an isogenic yeast strain expressing untagged Med15. Relative occupancy values were normalized to the average of 10 non-target regions.