Highly transcribed regions give higher signals in ChIP experiments.

<p>(A) Mediator association at the indicated <i>GAL</i> regions in cells grown in galactose (gal) or glucose (glu). Primer pair <i>GAL1</i>+976 (underlined) is identical to that used by Zhu et al. (2006). Values were normalized to an ORF-free region on chromosome V, which was defined as 1. (B) TBP and (C) Hsf1 association at the indicated <i>GAL</i> regions in cells grown in galactose (Gal) or a mixture of glucose and galactose (GalGlu). (D) TBP and (E) Gal4 association with the indicated <i>GAL</i> and <i>SSA4</i> regions in cells grown in absence (NHS) or presence of an 8 min heat shock (HS) at 39°C. (F) Mediator association with genes highly transcribed by Pol I (<i>RDN18</i> and <i>RDN37</i>) and Pol III (<i>RDN5</i>).