Lack of toxicity of transgenic shRNA expression.

<p>(A) Tet29 rats were treated with doxycycline (DOX) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005124#pone-0005124-g003" target="_blank">Figure 3</a>. At the end of the experiment, 20 µg of total RNA from liver was used in an RPA for detection of mir122. M: RNA Decade marker; Y: yeast RNA; Y-: yeast RNA without RNase digestion; nt: nucleotides. Protein kinase R (PKR) expression was used as marker for interferon response in white adipose tissue of acutely (B, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005124#pone-0005124-g001" target="_blank">Figure 1</a>) or in heart of chronically (C, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005124#pone-0005124-g003" target="_blank">Figure 3</a>) DOX treated wild-type (WT), Tet14, and Tet29 rats. PKR was detected by Western blot in 20 µg protein; an unspecific band (indicated by *) was used as loading control. HEKi: positive control, 20 µg of protein of HEK cells treated with 1 µM interferon-α2a for 24 hours.