The Sg4E-BP variant mimicking hyperphosphorylation inhibits protein synthesis in sea urchin cell-free cap-dependent translation system.

<p>Different amounts (20; 100; 200 or 1,000 ng) of recombinant proteins (GST, GST-Sg4E-BP WT (WT), GST-Sg4E-BP YALA (YALA), GST-Sg4E-BP 4xA (4xA), GST-Sg4E-BP 4xE (4xE)) were added to the fertilized cell-free translation system and Luciferase activity was measured as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005070#s4" target="_blank">Materials and Methods</a> after addition of the Cap+mRNA encoding Renilla Luciferase. The Luciferase activity is represented in RLU (Relative Light Units). Error bars represent the standard deviation (s.d.) of duplicates.