Mature BMDC inefficiently support Th17 differentiation.

<p>CD4⁺ T cells were cultured with immature or mature BMDC at a 2/1 CD4⁺/BMDC ratio in the presence of anti-CD3 under Th17 conditions. (A) BMDC were stimulated with LPS (1 µg/ml) and stained for CD40, CD86, or MHCII (IA/IE) expression. The mean fluorescence intensity are shown. Data are representative of one experiment out of three. (B) Cells were stained intracellularly for IL-17 and IFN-γ. Shown is one representative experiment out of 6. (C) Cumulative data of the percentage of CD4⁺IL-17 and IFN-γ positive T cells after restimulation as assessed by intracytoplasmic staining. Data represent the mean±SEM of 6 independent experiments. (D) The ratio of the percentage of IL-17⁺ to IFN-γ⁺ cells among CD4⁺ T cells. (E) CD4⁺ T cells (2.5×10⁵ cells) were cultured with immature or mature BMDC at three different CD4⁺/BMDC ratios (2/1; 1/1; 1/2) as described in A. Data represent the mean±SD of 4 independent experiments. P values were calculated using the two-tailed, paired Student's <i>t</i> test, *, P<0.05; **, P<0.01; ***, P<0.001.