Acute live cell binding in GH4-hα7 cells.

<p>Non-specific binding was determined in the presence of 10 µM methyllycaconitine (MLA). Data shown are the average±SEM of 3 separate experiments. A. Raw saturation binding data shows total, nonspecific, and specific binding for [¹²⁵I]α-BTX to α7-nAChR in this cell line. B. Specific binding of [¹²⁵I]α-BTX to α7-nAChR in fmol/mg protein with Scatchard analysis. C. Comparison of maximal specific [¹²⁵I]α-BTX binding displacement by AChE peptides T14 and T30, as compared with known α7-nAChR antagonists and agonists. ACh = acetylcholine. D. Maximal specific [¹²⁵I]α-BTX binding displacement by control peptides, full length T-AChE, and truncated AChE (T548). E. Displacement binding profiles for AChE C-terminal peptides T14 and T30.