Cysteine residues from predicted catalytic triads are essential for Cif homologs activity.

<p>(A) HeLa cells were treated with purified Cif homologs proteins (wild-type (WT) or cysteine variants (C/S)) in combination with a lipidic delivery agent (BioPORTER). F-actin was stained with phalloidin-rhodamine (red) and DNA with DAPI (blue) 72 h post-treatment. Bars represent 20 µm. (B) G₁/S synchronized HeLa cells were treated with PBS or purified Cif proteins (as indicated), in combination with BioPORTER. The percentages of the populations containing 4N DNA content were determined by flow cytometry 20 h post-treatment.