Increasing steady state levels of PPP1R11, PP1γ2 and Sds22 in testis parallel the temporal progression of spermiogenesis.

<p>In each experiment, postnatal testis levels of PP1γ2, Sds22, and PPP1R11 were monitored by assessing equal concentrations of protein (25 µg) from extracts prepared from each age group. Proteins were separated by SDS-PAGE, and gels were assayed by western blotting with antibodies against PP1γ2, Sds22, and PPP1R11. Results indicating that postnatal expression of PP1γ2, Sds22, and PPP1R11 increased over the age range in which the initial round of spermiogenesis takes place were visualized by chemiluminescence. A duplicate blot was probed with an anti-actin antibody to demonstrate equal protein loading.