Mammary tumor cells derived from adiponectin haplodeficient mice were more aggressive.

<p>Primary mammary tumor cells were isolated from FVB/N PyVT mice with normal [<i>PyVT(+/−)/ADN(+/+)</i>] or reduced [<i>PyVT(+/−)/ADN(+/−)</i>] adiponectin expressions, and implanted into nude mice for assessing their tumor development <i>in vivo</i> (A and B), or subjected to culture and [³H]-thymidine incorporation assays for evaluating their proliferations <i>in vitro</i> (C and D). The comparison between <i>PyVT(+/−)/ADN(+/+)</i> and <i>PyVT(+/−)/ADN(+/−)</i> groups were performed for tumor cells derived from both female (A and C) and male (B and D) mice. Tumor <i>g</i>rowth was presented as the fold changes of tumor volume against the first measurement at day 4 (A and B). DNA synthesis was monitored in 0.5% and 10% FBS culture conditions at 24 and 48 hrs after seeding (C and D). CPM, counts per minute. *, P<0.05 and **, P<0.01 vs corresponding groups (n = 13–18).