CyaA and ET promote c-maf and GATA-3 expression in primed T cells.

<p>Enriched CD4⁺ T cells from 3 healthy donors were primed with anti-CD3 mAb, as such or following pretreatment for 2 h with 0.28 nM CyaA or 0.11 nM ET. Cells primed in Th2- (IL-4) or Th1- (IL-12) inducing conditions were included as controls. After 10 days cells were restimulated with anti-CD3 mAb for 24 h. The levels of mRNA encoding c-maf and GATA-3 (A) and T-bet (B) were quantified by real-time RT–PCR. Transcript levels were normalized to the expression level of GAPDH. Syber green runs were performed with cDNAs from the same reverse transcription reaction from 400 ng of total RNA. The ΔΔC_T method was applied as a comparative method of quantification, using cells primed in neutral conditions (anti-CD3 mAb) as reference. The data are representative of 3 independent experiments, each in duplicate. ***<i>P</i>≤0.00001; **<i>P</i>≤0.0001; *<i>P</i>≤0.001. Error bars, SD.