Localization of PDE3B in isolated hepatocytes.

<p>Hepatocytes (35–100×10⁶) were isolated from C57BL/6 mice and subjected to subcellular fractionation. Four compartments were isolated; the plasma membranes (PM), internal membranes (IM), mitochondria (Mit) and Nuclei (Nucl). The fractions were subjected to PDE3 activity measurements (a) and immunoblot analysis (b) with antibodies against PDE3B, caveolin-1 (Cav-1, a caveolae marker), flotillin-1 (Flot-1, a lipid raft marker), Na⁺K⁺-ATPase (NaKA, a PM marker), BiP (ER marker) and nucleoporin p62 (Nucl, a nucleus marker). PDE3 activities are shown as mean±SEM and immunoblots as one representative experiment (n = 4). Amount of proteins loaded on the gel: PM, 79 µg; IM, 82 µg; Mitochondria, 31 µg; Nuclei, 31 µg.