Reduced apoptosis in myr-Akt1 parotid glands following exposure to ionizing radiation.

<p>In A, four-week old female FVB and myr-Akt1 mice were exposed to 0.5, 1, or 5 Gy γ-radiation and parotid salivary glands were removed 24 hours post-irradiation. Tissues were embedded into paraffin and sections were stained for activated caspase-3. The number of caspase-3 positive cells is graphed as a percentage of the total number of cells per field of view. In B, four-week old female FVB and myr-Akt1 mice were exposed to 1 Gy γ-irradiation and parotid salivary glands were removed at 24, 48, 72 and 96 hours. Tissues were processed for activated caspase-3 immunohistochemistry as described in A. Five fields of view were quantitated for each tissue section and graphed using the averages and SEM from three mice per group. Significant differences (p≤0.05) were determined using a two sample t-test comparing FVB to myr-Akt1 and significant differences are marked with an asterisk (*).