Triglyceride and cholesterol contents are increased in hepatocytes isolated from PDE3B KO mice.

<p>Hepatocytes (a, c–e) and liver (b) from PDE3B KO and WT mice were analyzed with regard to triglyceride (a) and cholesterol (b) content. Furthermore, PPARγ mRNA (c), SREBP1c mRNA (d) and HMG CoA reductase mRNA (e) were analyzed in hepatocytes. Values are means±SEM. Triglyceride content was analyzed as duplicate lipid extractions from four animals of each genotype, p<0.03. Cholesterol content was measured in duplicate in six mice of each genotype, p<0.03. mRNA expressions were analyzed in duplicate in two independent experiments from two mice of each genotype.