Analysis of the different pathways activated by Fas in HT29 and RHT29 cells.

<p>To determine the ability of Fas to induce cell death, cells were treated or not with A) oxaliplatin and B) MMP inhibitor for 24 hours, and then apoptosis was induced by the addition of the agonistic antibody CH11 at a final concentration of 150 ng/ml. Apoptosis induction by Fas activation was determined by annexin V/IP staining as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004728#s4" target="_blank">Materials and Methods</a>. Results are represented as arbitrary units assuming that control value is 1 and are mean±SEM for 3 samples per group. C) Activation of MAPK pathways in cells treated or not with 150 ng/ml of CH11 for 24 hours was detected by Western blot analysis. Tubulin expression was used as an endogenous control. Values that are significantly different between control and treated group by ANOVA analysis are indicated by *p<0.05,**p<0.001, ***p<0.0001. <i>OXA</i>: oxaliplatin.