LTP studies in NEP-deficient mice and their wild-type controls.

<p>LTP in the hippocampus (CA1 region) and the amygdala (lateral nucleus of the amygdala (LA)) in adult (n = 7 mice) and aged NEP knockout mice (−/−; n = 7 mice) in comparison to wild-type (+/+) mice (n = 5 mice in each group). (a) 9-month-old mice: LTP magnitude (CA1: ○ +/+, n = 9 slices; • −/−, n = 11 slices; LA: ○ +/+, n = 10 slices, • −/−, n = 11 slices). Inserted representative records of PS potentials in the CA1 region before and after TBS and in the LA before and after HFS, above: obtained from −/−, below: obtained from +/+. (b) 24-month-old mice: LTP in both the CA1 region (○ +/+, n = 11 slices; • −/−, n = 16 slices) and the LA (○ +/+, n = 11 slices; • −/−, n = 10 slices). Data points in a and b represent mean amplitudes (mean±s.e.m.) normalized with respect to baseline values. Inserted representative records of PS potentials in the CA1 region before and after TBS and in the LA before and after HFS, above: obtained from −/−, below: obtained from +/+. (c) Bar histogram of data points shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0004590#pone-0004590-g004" target="_blank">Figures 4a–b</a>, averaged 56 to 60 min after TBS/HFS and normalized with respect to baseline (mean±s.e.m.). *<i>P</i><0.05.