Loss of TAK1 in the intestinal epithelium leads to severe epithelial damage and upregulates inflammatory gene expression in response to DSS.

<p>A Histologic damage was scored from H&E staining sections of distal colon from TNFR1KO and TNFR1KO/TAK1^IEKO mice treated with water or 2.5% DSS for 5 days. Each bar represents a mean value±SE (N = 6 for water control; N = 9 for DSS, ** = p<0.01, NS = not significant). B Representative H&E staining. Scale bars, 100 µm. C Total RNA was isolated from distal colon of TNFR1KO and TNFR1KO/TAK1^IEKO mice at day 5 of DSS administration and reverse transcribed. The expression of inflammatory genes was measured using quantitative real-time PCR. Each bar represents a mean value±SE (N = 5 per group, ** = p<0.01).