Evidence supporting a direct effect of hA3G on relative infectivity of HIV-1 virions produced from Th1 and Th2 cells.

<p>In vitro cytokine-derived Th2 cells were transduced with a control lentiviral vector (Th2-Empty) or a vector expressing hA3G (Th2-A3G). Protein levels of hA3G packaged into virions produced from Th1, Th2, and hA3G-transduced Th2 cells were determined by Western Blotting on the LICOR Odyssey system (Relative Light Units (RLU) of hA3G as normalized by HIV-1 p24 antigen) Shown is a representative blot of three experiments with similar trends. (A). The infectivity of <i>vif</i>-deleted HIV-1(NL4-3) virions produced from those cells from 6 donors was determined using the TZM-bl indicator cell line. Error bars represent median and interquartile range (*p = .03). (B) In vitro cytokine-derived Th1 cells were activated and incubated with either an isotype control or neutralizing IFN-γ antibodies for 48 hours. Protein concentrations were determined by Western blot (C,open bars). The infectivity of <i>vif</i>-deleted HIV-1(NL4-3) virions produced from those cells was determined using the TZM-bl indicator cell line (C, closed bars).