Non-selective uptake via macropinocytosis of Lucifer Yellow (LY) in zebrafish kidney phagocytes.

<p>The <i>y</i>-axis represents fold increase in mean fluorescent intensity (MFI) compared to basal conditions (4°C treatment). The <i>x</i>-axis represents the experimental conditions during incubation. A) After an incubation time of 30 min a significant uptake of LY at 30°C and 37°C was observed. B) The inhibition affect of Cytochalasin D (CCD) (2.5 µg/ml) when added to kidney cells prior to incubation for 1 hour with LY. Addition of the inhibitor EDTA (1 mM) had no significant effect on LY uptake in granulocytes and macrophages. C) Macropinocytosis in zebrafish lymphocyte population. Non-selective uptake of LY was also observed in cells of the lymphocyte gate. This uptake was significantly inhibited by CCD (2.5 µg/ml) but not by EDTA (1 mM). Same letters indicate no significant difference in MFI. Average fold change in MFI±s.e.m. from 3 replicates is shown (<i>p</i><0.05).