In-vivo cytokine production in LDLR+/+ and LDLR−/− mice infected with <i>S. typhimurium</i>.

<p>LDLR−/− and control (LDLR+/+) C57Bl/6J mice were infected i.v. with 10² cfu of <i>S. typhimurium</i>. After 1, 3 or 7 days, groups of 5 mice/group were sacrificed, and circulating concentrations of cytokines in the plasma were measured by specific ELISA. In a separate experiment, TNF and IL-1β mRNA in the spleens of LDLR+/+ and LDLR−/− mice was assessed by semi-quantitative RT-PCR, and expressed as ratio to GAPDH mRNA expression. Data are presented as means±SD, *p<0.05.