Accumulation of Notch and Delta proteins in clones of ESCRT-II mutants.

<p>(A,B,C) Areas of increased Ubiquitin (Ub) immunoreactivity frequently co-localize with areas containing accumulated levels of the Notch receptor (antibody against the extracellular domain of Notch, N[extra]) in ESCRT-II mutant tissue. GFP/Ubiquitin/N^extra (green/red/blue) co-labelings of (A) <i>vps22^5F8-3</i>, (B) <i>vps25^N55</i> and (C) <i>vps36^L5212</i> eye mosaics. Scale bars represent 50 µm. (A′,B′,C′) Ubiquitin/N^extra (red/blue) co-labeling of (A′) <i>vps22^5F8-3</i>, (B′) <i>vps25^N55</i> and (C′) <i>vps36^L5212</i> eye mosaics. (A″,B″,C″) Ubiquitin labeling of (A″) <i>vps22^5F8-3</i>, (B″) <i>vps25^N55</i> and (C″) <i>vps36^L5212</i> eye mosaics. (A′″,B′″,C′″) N^extra labeling of (A′″) <i>vps22^5F8-3</i>, (B′″) <i>vps25^N55</i> and (C′″) <i>vps36^L5212</i> eye mosaics. (D,E,F) Accumulation of Notch protein using an antibody detecting the intracellular domain of Notch, N[intra]). GFP/N^intra (green, red) co-labelings of (D) <i>vps22^5F8-3</i>, (E) <i>vps25^N55</i> and (F) <i>vps36^L5212</i> eye mosaics. Scale bars represent 100 µm (D′,E′,F′) N^intra (red) labeling of (D′) <i>vps22^5F8-3</i>, (E′) <i>vps25^N55</i> and (F′) <i>vps36^L5212</i> eye mosaics. (G,H,I) The Notch ligand Delta accumulates in clones of ESCRT-II mutants. GFP/Delta (green/magenta) co-labeling of (G) <i>vps22^5F8-3</i>, (H) <i>vps25^N55</i> and (I) <i>vps36^L5212</i> eye mosaics. Scale bars represent 100 µm (G′,H′,I′) Delta labeling of (G′) <i>vps22^5F8-3</i>, (H′) <i>vps25^N55</i> and (I′) <i>vps36^L5212</i> eye mosaics. Genotypes: (A,D,G) <i>eyFlp</i>; <i>FRT82B vps22^5F8-3/FRT82B P[ubi-GFP]</i>. (B,E,H) <i>eyFlp</i> ; <i>FRT42D vps25^N55/FRT42D P[ubi-GFP]</i>. (C,F,I) <i>eyFlp</i> ; <i>vps36^L5212 FRT2A/P[ubi-GFP] FRT2A</i>.