Apoptosis phenotype of ESCRT-II mutants.

<p>Labeling of <i>vps22</i>, <i>vps25</i> and <i>vps36</i> eye-antennal imaginal discs with cleaved Caspase-3 antibody as apoptotic marker. Arrows in (A′), (B′) and (C′) point to one representative clone in each panel containing increased caspase-3 activity. (A–C) GFP/Caspase-3 (green/red) co-labelings of (A) <i>vps22^5F8-3</i>, (B) <i>vps25^N55</i> and (C) <i>vps36^L5212</i> eye mosaics. (A′–C′) Caspase-3 labeling (red) of (A′) <i>vps22^5F8-3</i>, (B′) <i>vps25^N55</i> and (C′) <i>vps36^L5212</i> eye mosaics. Genotypes: (A) <i>eyFlp</i> ; <i>FRT82B vps22^5F8-3/FRT82B P[ubi-GFP]</i>. (B) <i>eyFlp</i> ; <i>FRT42D vps25^N55/FRT42D P[ubi-GFP]</i>. (C) <i>eyFlp</i> ; <i>vps36^L5212 FRT2A/P[ubi-GFP] FRT2A</i>.