Effects of long-circulating polyethylene glycol (PEG) minocycline-liposomes on the clinical course of experimental autoimmune encephalomyelitis (EAE).

<p>A single intravenous (i.v.) injection of PEG minocycline-liposomes given after disease onset at day 15 post-immunization resulted in significant amelioration of clinical disease for eight days compared to a single injection of PBS when given shortly after onset of clinical disease (A). This effect was not sustained (A). In subsequent experiments, PEG-liposomes were administered every five days. Treatment with i.v. PEG minocycline-liposomes initiated after disease onset at day 15 post-immunization and administered every five days was as effective in ameliorating clinical EAE as treatment with daily intraperitoneal (i.p.) injections of minocycline (B). In contrast, i.v. injections of empty PEG-liposome every five days, or minocycline i.p. injections every five days had no detectable effect on the clinical course of EAE (B). The time of treatment initiation is indicated by a red arrow.