MiR-221 is strongly expressed in prostate carcinoma-derived primary cells and its expression inversely correlates with that of p27.

<p>A MiR-221 and miR-222 expression measured by quantitative real-time PCR in primary cell lines from prostate carcinomas (T samples) or normal prostate (N samples). The graph shows the log fold change of miR-221 and miR-222 expression as compared to the value obtained for non-tumoral control sample N1. B Representative Western blot analysis showing p27 expression in 4 tumor- and 2 non-tumor-derived primary cell lines. β-actin immunoreactivity is shown as a loading control. Under each lane, the numerical values represent p27 relative amount, that was set = 1 in the normal control sample N1. C Spearman correlation analysis performed between miR-221, miR-222, and p27 levels in 18 primary cell lines derived from prostate carcinoma tissues. P<0.05.